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Senior Research Associate - Protein Expression & Purification

2.00 to 6.00 Years   Bangalore, Chennai, Hyderabad, Kolkata   21 Dec, 2021
Syngene International Limited
Job LocationBangalore, Chennai, Hyderabad, Kolkata
EducationNot Mentioned
SalaryNot Disclosed
IndustryEducation / Training
Functional AreaR&D / Product Design
EmploymentTypeFull-time

Job Description

Designation: Senior Research Associate - Protein Expression & PurificationJob Location: BengaluruDepartment: Discovery BiologyAbout SyngeneIncorporated in 1993, Syngene International Ltd. is an innovation-focused global discovery, development and manufacturing organization providing integrated scientific services to the pharmaceutical, biotechnology, nutrition, animal health, consumer goods and specialty chemical industries around the world. Syngene s clientele includes world leaders such as Bristol-Myers Squibb, Baxter, Amgen, GSK, Merck KGaA and Herbalife. Its innovative culture is driven by the passion of its 4240- strong team of scientists who work with clients from around the world to solve their scientific problems, improve R&D productivity, speed up time to market and lower the cost of innovation.Job Purpose:

  • The incumbent will perform protein expression and purification from various expression systems.
  • Key Responsibilities:
  • Gene cloning, protein expression involving E.coli, & Mammalian cell lines (Insect cell work is highly appreciated) and purification involving small & large scales of batch sizes (2L 20L).
  • Experience with tagged and non-tagged genes for expression and purification, involving expertise in hands-on ion-exchange chromatography, hydrophobic ion chromatography, size-exclusion chromatography, analysis by SDS-PAGE & Western blots, ELISA assays.
  • The candidate should be independently able to purify a protein in a multi-step purification (~ 2-5 steps) and have negligible endotoxin amounts.
  • The candidate should maintain up-to date lab note books at the time of completing the experiment.
  • Ability to work in a team is important and the candidate should demonstrate this objective.
  • Understanding and demonstrating responsibility for safety of self & colleagues by following all safety protocols provided by Syngene
  • Adherence to all environment, health, and safety (EHS) policies & standards at all times in the workplace
  • Wearing all PPE as required.
  • Adherence to all procedures related to Syngene s data integrity policies
  • Compliance to Syngene s quality standards at all times.
Educational Qualification:M.Sc/M.Tech in Biotechnology or related disciplinesTechnical/functional Skills: Expert level in protein expression and purification. Should be conversant with SDS-PAGE gels, western blots, chromatographic modes of purification, understanding of protein analytical data. Maintaining Lab notebooks.Experience:2-6 years post qualificationBehavioral Skills:Should be well versed in verbal and written communication, should be a team player. Should be focused towards results and deliverables. Should have a high moral framework (ethics code) and high data integrity sense.Equal Opportunity Employer:It is the policy of Syngene to provide equal employment opportunity (EEO) to all persons regardless of age, color, national origin, citizenship status, physical or mental disability, race, religion, creed, gender, sex, sexual orientation, gender identity and/or expression, genetic information, marital status, status with regard to public assistance, veteran status, or any other characteristic protected by applicable legislation or local law. In addition, Syngene will provide reasonable accommodations for qualified individuals with disabilities.,

Keyskills :
equal employment opportunitynmrprotein expressionhplcquality standardsresearchcell linesanimal healthconsumer goodsliteraturedata integrityvalidationcontinuous improvement facilitation

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