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Associate Scientist-Protien Expression

4.00 to 5.00 Years   Chennai   09 Dec, 2021
Sygene
Job LocationChennai
EducationNot Mentioned
SalaryNot Disclosed
IndustryPharma / Biotech
Functional AreaGeneral / Other Software
EmploymentTypeFull-time

Job Description

Designation: Associate Scientist-Protein ExpressionJob Location: BangaloreDepartment: Discovery BiologyAbout SyngeneIncorporated in 1993, Syngene International Ltd. is an innovation-focused global discovery, development and manufacturing organization providing integrated scientific services to the pharmaceutical, biotechnology, nutrition, animal health, consumer goods and specialty chemical industries around the world. Syngene s clientele includes world leaders such as Bristol-Myers Squibb, Baxter, Amgen, GSK, Merck KGaA and Herbalife. Its innovative culture is driven by the passion of its 4240- strong team of scientists who work with clients from around the world to solve their scientific problems, improve R&D productivity, speed up time to market and lower the cost of innovation.Job Purpose:The incumbent will perform protein expression and purification from various expression systems, following ALCOA+ principles and Data integrity policy.Key Responsibilities:

  • Protein expression involving E.coli, & Mammalian cell lines (Insect cell work is highly appreciated) and purification involving small & large scales of batch sizes (2L 20L).
  • Experience with tagged and non-tagged genes for expression and purification, involving expertise in hands-on ion-exchange chromatography, hydrophobic ion chromatography, size-exclusion chromatography, analysis by SDS-PAGE & Western blots, ELISA assays.
  • The candidate should be independently able to purify a protein in a multi-step purification (~ 2-5 steps) and have negligible endotoxin amounts.
  • The candidate should maintain up-to date lab note books at the time of completing the experiment.
  • Ability to work in a team is important and the candidate should demonstrate this objective.
  • Understanding and demonstrating responsibility for safety of self & colleagues by following all safety protocols provided by Syngene
  • Adherence to all environment, health, and safety (EHS) policies & standards at all times in the workplace
  • Wearing all PPE as required.
  • Adherence to all procedures related to Syngene s data integrity policies
  • Compliance to Syngene s quality standards at all times.
Educational Qualification: Candidate with a MSc/MTech in Life sciencesTechnical/functional Skills:
  • Carry out DNA scale up and protein expression as per the requirements of the specific job function.
  • Protein expression function to include ability to reliably and reproducibly express.
  • Proteins in one or more expression systems including mammalian, insect cells.
  • Protein expression skills should include knowledge and experience in the operation of wave bioreactors and incubator shakers.
  • Adhere to timely and accurate record keeping in lab notebooks.
Experience:6-9 Post qualificationBehavioral Skills:
  • Take accountability and ownership for respective job function maintain data integrity & exhibit good data management skills.
  • Excellent attention to details, diligent documentation and efficient communication skills
Equal Opportunity Employer:It is the policy of Syngene to provide equal employment opportunity (EEO) to all persons regardless of age, color, national origin, citizenship status, physical or mental disability, race, religion, creed, gender, sex, sexual orientation, gender identity and/or expression, genetic information, marital status, status with regard to public assistance, veteran status, or any other characteristic protected by applicable legislation or local law. In addition, Syngene will provide reasonable accommodations for qualified individuals with disabilities.,

Keyskills :
hplcrecord keepinganimal healthequal employment opportunitysopdocumentationconsumer goodsdata integritycell linesvalidationprotocolscontinuous improvement facilitationpolymerase chain reaction pcr

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