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Research Investigator-Protein Purification

0.00 to 3.00 Years   Delhi   10 Mar, 2021
Sygene
Job LocationDelhi
EducationNot Mentioned
SalaryNot Disclosed
IndustryPharma / Biotech
Functional AreaBio Tech / R&D / Scientist
EmploymentTypeFull-time

Job Description

  • We are seeking a skilled and enthusiastic protein biochemist at a Research Investigator level to join the Protein Purification group.
Key Responsibilities:
  • To perform mammalian and microbial (desirable) recombinant protein purification techniques (including affinity, ion exchange, HIC, SEC, CHT and reverse phase chromatography, TFF) and methods development. Candidates having demonstrated hands-on experience in both mammalian and microbial projects will be preferred.
  • Should have experience in analytical biochemistry (SEC-HPLC, SEC-MALS/DLS, SDS PAGE, Western Blot, Protein quantification etc.)
  • Strong FPLC/liquid chromatography experience ability to program, operate, and troubleshoot purification equipment and experiments at small/pilot scale using the GE AKTA platform including AKTA PURE, Pilot, Explorer.
  • Possess the knowledge and exposure to environment, health, and safety (EHS) practices.
  • Follow environment, health, and safety (EHS) requirements at all times in the workplace ensuring individual and lab/plant safety.
  • Ensure environment, health, and safety (EHS) measures are imparted via training and adhered within the team.
Educational Qualification:
  • PhD in biotechnology, biochemistry, structural biology, biophysics, bio-analytical chemistry or similar scientific fields.
Technical/functional Skills:
  • Ability to learn new techniques, perform multiple tasks simultaneously, update technical data and maintain accurate records contemporaneously, follow instructions, and comply with company policies.
  • Work according to established Standard Operating Procedure (SOP) and regulatory guidance.
  • Should have excellent oral, presentation and written communication skills: Team player and customer-focused
  • Should have good publication record.
  • Apply mass spectrometry - based approaches, including intact mass, glycan profiling, glycosylation site and disulfide bond mapping, peptide mapping to support protein analysis and characterization.
  • Additional advantage is having a demonstrated experience in optimizing expression and purification of recombinant proteins from microbial source.
  • Knowledge and experience in packing resins in columns like XK, HiScale and BPG.
  • Knowledge in moderate throughput purification techniques (e.g-Magbeads, Coupled Columns) is desirable
Experience:
  • 0-3 years of experience post qualification. Industrial experience is an advantage.
Behavioural Skills:
  • Excellent time management and organizational skills, and attention to detail.
  • Ability to work independently and as part of a team, self-motivation, adaptability.
  • Must have a high moral framework (ethics code) and high sense of data integrity values.
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Keyskills :
process safetyaccountabilitycgmpequal employment opportunitystandard operating procedureapicontinuous improvement facilitationprocess development

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