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Associate Scientist/Senior Associate Scientist-Protien Expression

6.00 to 9.00 Years   Mumbai City   06 Oct, 2020
Syngene International Limited
Job LocationMumbai City
EducationNot Mentioned
SalaryNot Disclosed
IndustryEducation / Training
Functional AreaBio Tech / R&D / Scientist
EmploymentTypeFull-time

Job Description

  • The candidate needs to be well trained in routine mammalian cell culture practices like establishment of cell banks, expansion and scale up of cell lines, serum free adaptation and characterization of cell lines. The Candidate should also have basic molecular biology experience.
Key Responsibilities:
  • Under the guidance of reporting manager design, plan and execute experiments along with compilation and analysis of data with minimal error.
  • Perform transient expressions in mammalian cell lines like HEK, CHO
  • Plan and perform protein expression at different scales like microtitre plates , shake flask and bioreactor
  • Perform optimization of media /Feed to improve productivity in batch and fed-batch culture
  • Perform serum free adaptations and express proteins in batch /fed batch mode using stable clones
  • Scale up plasmid DNA for transfection depending on the scale of the expression batch
  • Analysis of expression titers using OCTET, PA-HPLC , western blot
  • Perform small scale purification using affinity chromatography
  • Follow environment, health, and safety (EHS) requirements at all times in the workplace ensuring individual and lab/plant safety
  • Attend training on environment, health, and safety (EHS) measures imparted company
Educational Qualification: Candidate with a MSc/MTech in Life sciencesTechnical/functional Skills:
  • Carry out DNA scale up and protein expression as per the requirements of the specific job function.
  • Protein expression function to include ability to reliably and reproducibly express.
  • Proteins in one or more expression systems including mammalian, insect cells.
  • Protein expression skills should include knowledge and experience in the operation of wave bioreactors and incubator shakers.
  • Adhere to timely and accurate record keeping in lab notebooks.
Experience:6-9 Post qualificationBehavioral Skills:
  • Take accountability and ownership for respective job function maintain data integrity & exhibit good data management skills.
  • Excellent attention to details, diligent documentation and efficient communication skills
,

Keyskills :
marketingquality controlresearchauditingbenefits administrationmammalian cell cultureequal employment opportunitycontinuous improvement facilitationpolymerase chain reaction pcr

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