Associate Scientist

Job Description

Designation: Associate Scientist/ Senior Associate Scientist-Protein ExpressionJob Location: BangaloreDepartment: Discovery BiologyAbout SyngeneIncorporated in 1993, Syngene International Ltd. is an innovation-focused global discovery, development and manufacturing organization providing integrated scientific services to the pharmaceutical, biotechnology, nutrition, animal health, consumer goods and specialty chemical industries around the world. Syngene s clientele includes world leaders such as Bristol-Myers Squibb, Baxter, Amgen, GSK, Merck KGaA and Herbalife. Its innovative culture is driven by the passion of its 4240- strong team of scientists who work with clients from around the world to solve their scientific problems, improve R&D productivity, speed up time to market and lower the cost of innovation.Job Purpose:The candidate needs to be well trained in routine mammalian cell culture practices like establishment of cell banks, expansion and scale up of cell lines, serum free adaptation and characterization of cell lines. The Candidate should also have basic molecular biology experience.Key Responsibilities:
• Perform purification from bacterial host. protein expression involving E.coli (Insect cell work is highly appreciated) and purification involving small & large scales of batch sizes (2L 20L).
• Candidate should perform transformation and screening of recombinant protein expression.
• Perform optimization of culturing conditions to improvise the yield.
• To handle multiple client projects under supervision.
• Experience with tagged and non-tagged genes for expression and purification, involving expertise in hands-on ion-exchange chromatography, hydrophobic ion chromatography, size-exclusion chromatography using AKTA system, analysis by SDS-PAGE & Western blots, ELISA assays.
• The candidate should be independently able to purify a protein in a multi-step purification (~ 2-5 steps) and have negligible endotoxin amounts.
• The candidate should maintain up-to date lab note books at the time of completing the experiment.
• Ability to work in a team and communication is important and the candidate should demonstrate this objective.
• Follow environment, health, and safety (EHS) requirements at all times in the workplace ensuring individual and lab/plant safety
• Attend training on environment, health, and safety (EHS) measures imparted company
Educational Qualification: Candidate with a MSc/MTech in Life sciencesTechnical/functional Skills:Carry out DNA scale up and protein expression as per the requirements of the specific job function.Protein expression function to include ability to reliably and reproducibly express.Proteins in one or more expression systems including mammalian, insect cells.Protein expression skills should include knowledge and experience in the operation of wave bioreactors and incubator shakers. Adhere to timely and accurate record keeping in lab notebooks.Experience:6-9 Post qualificationBehavioral Skills:Take accountability and ownership for respective job function maintain data integrity & exhibit good data management skills.Excellent attention to details, diligent documentation and efficient communication skillsEqual Opportunity Employer:It is the policy of Syngene to provide equal employment opportunity (EEO) to all persons regardless of age, color, national origin, citizenship status, physical or mental disability, race, religion, creed, gender, sex, sexual orientation, gender identity and/or expression, genetic information, marital status, status with regard to public assistance, veteran status, or any other characteristic protected by applicable legislation or local law. In addition, Syngene will provide reasonable accommodations for qualified individuals with disabilities.,

Keyskills :
protocolshplcdocumentationcontinuous improvement facilitationanimal healthvalidationequal employment opportunitycell linesrecord keepingmammalian cell culturesopcell culture