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Associate Scientist

3.00 to 5.00 Years   Delhi   22 Jan, 2021
Syngene International Limited
Job LocationDelhi
EducationNot Mentioned
SalaryNot Disclosed
IndustryEducation / Training
Functional AreaBio Tech / R&D / Scientist
EmploymentTypeFull-time

Job Description

The incumbent will perform protein expression and purification from various expression systems, following ALCOA+ principles and Data integrity policy.Key Responsibilities:

  • Protein expression involving E.coli, & Mammalian cell lines (Insect cell work is highly appreciated) and purification involving small & large scales of batch sizes (2L 20L).
  • Experience with tagged and non-tagged genes for expression and purification, involving expertise in hands-on ion-exchange chromatography, hydrophobic ion chromatography, size-exclusion chromatography, analysis by SDS-PAGE & Western blots, ELISA assays.
  • The candidate should be independently able to purify a protein in a multi-step purification (~ 2-5 steps) and have negligible endotoxin amounts.
  • The candidate should maintain up-to date lab note books at the time of completing the experiment.
  • Ability to work in a team is important and the candidate should demonstrate this objective.
  • Follow environment, health, and safety (EHS) requirements at all times in the workplace ensuring individual and lab/plant safety
  • Attend training on environment, health, and safety (EHS) measures imparted company
Educational Qualification: Candidate with a MSc/MTech in Life sciencesTechnical/functional Skills:
  • Carry out DNA scale up and protein expression as per the requirements of the specific job function.
  • Protein expression function to include ability to reliably and reproducibly express.
  • Proteins in one or more expression systems including mammalian, insect cells.
  • Protein expression skills should include knowledge and experience in the operation of wave bioreactors and incubator shakers.
  • Adhere to timely and accurate record keeping in lab notebooks.
Experience:6-9 Post qualificationBehavioral Skills:
  • Take accountability and ownership for respective job function maintain data integrity & exhibit good data management skills.
  • Excellent attention to details, diligent documentation and efficient communication skills
,

Keyskills :
validationhplcsopdocumentationprotocolsequal employment opportunitycontinuous improvement facilitationcell linesanimal healthpolymerase chain reaction pcr

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